Since then the problem has received a lot of attention, and typical stateoftheart methods. In a more traditional definition the resolution limit is determined to the shape of the pointspread function of the instrument. Smith1,2, and katsumasa fujita1,2, 1department of applied physics, and 2immunology frontier research center, osaka university, suita, osaka, japan towhom correspondence should be addressed. Rapidly evolving techniques are continually altering the perspective of this emerging field with regards to imaging in cell biology.
Superresolution imaging department of image processing. They can also be used as tools for medical diagnostics and basic research. Zeiss microscopy online campus superresolution microscopy. Superresolution sr technique reconstructs a higherresolution image or sequence from the. Review introduction to superresolution microscopy masahito yamanaka1,2, nicholas i. For imaging, slices were transferred on their glass coverslip to a heated and perfused imaging chamber 32. If weights in each layer are forced to share their values, operations needed for features above the dashed line can be reused for each new frame. Related approaches acquire multiple sets of lowreslution highsnr images. New applications of superresolution in medical imaging. Efficient and accurate mri superresolution using a. Also included is an original article introducing the concept of expansion microscopy, which enables users to achieve superresolution using nonsuperresolution imaging platforms, a novel concept. Alpacas, llamas and camels, however, possess simpler antibodies that lack light chains and. Mri, superresolution, image enhancement, deep learning.
The braces in a,b mark the lower retinal slab shown in c containing the. Merge solutions facilitate the sharing of images to create a more effective and efficient electronic healthcare experience for patients and physicians. Bayesian combination of sparse and non sparse priors in image superresolution,digital signal processing, vol. Molecular model of fission yeast centrosome assembly.
Consensus equilibrium framework for superresolution and. Research on image statistics suggests that image patches can be wellrepresented as a sparse linear combination of elements from. Realtime video superresolution with spatiotemporal. Superresolution imaging in biomedicine ebook, 2017. Superresolution sr is the process of combining a sequence of lr images in order to produce an image or sequence of higher resolution. Simultaneous superresolution and crossmodality synthesis of 3d medical. How to extract 3d models of the human structure with highresolution images while reducing the level of radiation still remains a challenge 2, 3. The perfusion medium artificial cerebrospinal fluid, acsf consisted of 125 mm nacl, 2. Introduction radar imaging of rotating objects has been an area of interest for more than a decade, and several descriptions of the techniquessystems involved are available in the literature 15.
Superresolution imaging sr is a class of techniques that enhance increase the resolution of an imaging system. In this particular case, how does one obtain and combine different low. Superresolution imaging digital imaging and computer. Mri superresolution using selfsimilarity and image priors. Live cell superresolution requires trading off resolution, speed, and light dose. Image synthesis and superresolution in medical imaging.
Antibodies not only protect humans and other animals against diseasecausing bacteria and viruses. The matlab application implements the superresolution methods described in the following papers, developed for the present project. At the end of the article, we also discuss perspectives about the future development of superresolution microscopes and related techniques. These techniques combine sets of low resolution images to obtain images with. Image superresolution via sparse representation jianchao yang, student member. This paper provides an overview on superresolution sr research in medical imaging applications. A volumetric sdoct scan set is a collection of azimuthally sampled bscans a, creating a 3d view of the retina b. Recently, there has been a substantial effort to develop superresolution restoration techniques for medical imaging, such as xray mammography, functional magnetic. After this introduction, we describe the principles of several types of superresolution microscopes and their imaging properties and applications. In some cases, the acquired lowresolution images have to be upsampled to match with other highresolution images for posterior analysis or postprocessing such as registration or multimodal segmentation. Deep learning for medical image super resolution and segmentation. Imaging beyond these limits in medical imaging is referred to as super resolution. Introduction to superresolution microscopy the broad range of superresolution techniques available today are moving optical imaging of biological specimens into the realm traditionally held by electron microscopy. Higher factors of superresolution have been obtained by repeated application of this process.
However for sr, the original image undergoes three degradations during the measurement. Superresolution imaging in live cells sciencedirect. Superresolution ultrasound for imaging and microscopy conference paper pdf available in proceedings of the ieee ultrasonics symposium 3. Walter schempp holographic and superresolution imaging, proc. The diffractionlimited resolution of conventional light microscopy. Up to now, many sr methods have been studied and proposed. The requirements for better resolution in all medical imaging modalities. Conventional antibodies consist of light and heavy protein chains, and both are required to bind to target molecules or antigens. Two approaches are presented in this paper to improve the quality of digital images over the sensor resolution using superresolution techniques. Lowcost superresolution algorithms implementation over a hwsw video compression platform.
Superresolution sr is the process of combining a sequence of low resolution images in order to produce a higher resolution image or sequence we assume a similar acquisition model as in the case of multichannel blind deconvolution. Pdf on jan 1, 2017, m dirk robinson and others published new applications of superresolution in medical imaging find, read and cite all the research you need on researchgate. In physicsbased image synthesis in mr imaging, several images are acquired of the same anatomy with different pulse sequences and pulse sequence parameters so that one or more underlying physical parameterse. Superresolution imaging of hiv in infected cells with. Some techniques, such as those that rely of structured illumination or other optical effects such as tirf, can be applied to almost any fluorescently labeled sample, but provide limited resolution and cannot image single molecules. This case is equivalent to using 3d convolutions c, where the temporal information is merged with convolutions in space and time. The most significant advances in superresolution imaging have been achieved in what is termed farfield microscopy and involve either spatially or temporally modulating the transition between two molecular states of a fluorophore such as switching between a dark and bright state or by physically reducing the size of the pointspread function.
Spie 51, digital image synthesis and inverse optics. Each detailed chapter provides coverage of the implementations and applications of superresolution imaging. Pdf superresolution ultrasound for imaging and microscopy. Smallkernel superresolution methods for microscanning imaging systems jiazheng shi, stephen e. Extensive cytological, molecular, and genetic characterization of the s. If youre looking for a free download links of superresolution imaging digital imaging and computer vision pdf, epub, docx and torrent then this site is not for you.
Bigansst for perceptual image superresolution xiaotong luo 1, rong chen, yuan xie2, yanyun qu. Microscanning creates multiple lowresolution images. The sr task is an illposed problem, where one lr image has many solutions for hr image. Pdf multiframe superresolution reconstruction with. The key objective of superresolution sr imaging is to reconstruct a higherresolution image based on a set of images, acquired from the same scene and denoted as lowresolution images, to overcome the limitation andor illposed conditions of the image acquisition process for facilitating better content visualization and scene recognition. Superresolution imaging of cell structures can achieve a resolution of tens of nm. Superresolution in medical imaging the computer journal. Pdf new applications of superresolution in medical imaging. Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude. Its 14 sections span a wide range of modern superresolution imaging techniques and includes variational, bayesian, featurebased, multichannel, learningbased. In optical sr the diffraction limit of systems is transcended, while in geometrical sr the resolution of digital imaging sensors is enhanced in some radar and sonar imaging applications e.
Using geneticallyencoded fluorescent tags or small molecule dyes, superresolution imaging makes it possible to visualize structures much smaller than the diffraction limit of traditional optical microscopy techniques, allowing investigators to. The most common estimation framework begins with a cost function or penalty function relating. Welcome to allele biotechnologys superresolution imaging portal superresolution imaging is one of the fastest growing areas of biological research. Superresolution imaging of the extracellular space in.
Smallkernel superresolution methods for microscanning. Channel splitting network for single mr image superresolution. They used the frequency domain approach to demonstrate the ability to. The goal of superresolution image processing is to estimate the highresolution image x from the set of captured images. Superresolution from a single image methods for superresolution sr can be broadly classified into two families of methods. Pdf on jan 1, 2017, m dirk robinson and others published new. Felix koberling for the purchase of this volume in printed format, please visit. Single molecule spectroscopy and superresolution imaging. In magnetic resonance imaging typical clinical settings, both low and highresolution images of different types are routinarily acquired. In the early 1980s, tsai 2 has addressed the topic of image superresolution for the. Some provide anatomical information and reveal information about the structure of the human body, and others provide functional information, locations of activity for specific activities and specified tasks. Advances and challenges in superresolution sina farsiu, 1dirk robinson, michael elad,2 peyman milanfar1 1 electrical engineering department, university of california, santa cruz ca 95064 2 computer science department, the technionisrael institute of technology, israel received 30 january 2004. Pdf new applications of superresolution in medical.
Then, we will combine the loss terms in different ways to. Given the underlying physical parameters, these same imaging equations can. Download citation superresolution in medical imaging this paper. By infusing blood vessels with gasfilled microbubbles and using rapid ultrasound imaging to detect the bubbles, superresolution imaging of an entire vessel system has. However, detailed molecular understanding of additional mtocs in highly tractable genetic organisms is important to elucidate broad principles of assembly and regulation. Superresolution reconstruction produces one or a set. Super resolution imaging for better medical image analysis. Medical imaging ct, mri, ultrasound etc59, 70, 47, 60.
The resolving power of an imageforming instrument is limited by the highest spatial frequency transmitted by the system. The purpose of most radiologic imaging examinations is to either identify abnormalities in a patient or to classify a patient as being healthy while minimizing radiation exposure. Others, such as those described below, allow imaging of single molecules, but require careful choice of an optimal. Imaging protein assemblies at molecular resolution without affecting biological function is a longstanding goal. To achieve these goals, it is of obvious importance to ensure that the radiologic image quality is sufficient for a given imaging task. Single molecule spectroscopy and superresolution imaging ix editors. Translation microscopy tram for superresolution imaging. As for medical imaging, within each imaging modality, specific physical laws are in control, defining the meaning of noise and the sensitivity of the imaging process. Superresolution microscopy reveals unprecedented detail of immune cells surface 15 june 2016 salk scientists used lightsheet superresolution imaging. Journal of medical imaging journal of micronanolithography, mems, and moems. However, classical interpolation techniques are not.
This book encompasses the full breadth of the superresolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in. Multiframe superresolution reconstruction with applications to medical imaging thesis pdf available january 2018 with 212 reads how we measure reads. From the earliest algorithm proposed by tsai and huang, 12 superresolution has attracted a growing interest as a purely computational means to increase imaging sensors performance. Restoration of a single superresolution image from several.
492 1162 960 1260 518 122 1314 1327 1274 9 1344 1012 667 802 572 695 1325 1049 562 1227 1428 408 1506 1167 824 1025 239 1265 1244 932 871 1032 399 397 217 728 510 202 184 1432 173 271 1467